SWATH analysis of Yeast Proteome over time in response to Osmotic Stress. We sampled cell cultures in biological triplicates at six time points following the application of osmotic stress and acquired single injection DIA datasets on a high-resolution 5600 tripleTOF instrument operated in SWATH mode. Proteins were quantified by the targeted extraction and signal integration from the SWATH-MS datasets of peak groups representing proteotypic peptides for specific yeast proteins.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: yeast ; SWATH-MS ; 5600 ; osmotic stress ; MassIVE.quant reviewed - Platinum
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Ruedi Aebersold, Institute of Molecular Systems Biology, ETH Zurich, N/A |
Submitting User: | ccms |
Selevsek N, Chang CY, Gillet LC, Navarro P, Bernhardt OM, Reiter L, Cheng LY, Vitek O, Aebersold R.
Reproducible and consistent quantification of the Saccharomyces cerevisiae proteome by SWATH-mass spectrometry.
Mol. Cell Proteomics. 2015 Mar;14(3):739-49. Epub 2015 Jan 5.
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