Raw file Experiment Enzyme Enzyme mode Enzyme first search Enzyme mode first search Use enzyme first search Variable modifications Fixed modifications Multi modifications Variable modifications first search Use variable modifications first search Requantify Multiplicity Max. missed cleavages Labels0 LC-MS run type Time-dependent recalibration MS MS/MS MS3 MS/MS Submitted MS/MS Submitted (SIL) MS/MS Submitted (ISO) MS/MS Submitted (PEAK) MS/MS Identified MS/MS Identified (SIL) MS/MS Identified (ISO) MS/MS Identified (PEAK) MS/MS Identified [%] MS/MS Identified (SIL) [%] MS/MS Identified (ISO) [%] MS/MS Identified (PEAK) [%] Peptide Sequences Identified Peaks Peaks Sequenced Peaks Sequenced [%] Peaks Repeatedly Sequenced Peaks Repeatedly Sequenced [%] Isotope Patterns Isotope Patterns Sequenced Isotope Patterns Sequenced (z>1) Isotope Patterns Sequenced [%] Isotope Patterns Sequenced (z>1) [%] Isotope Patterns Repeatedly Sequenced Isotope Patterns Repeatedly Sequenced [%] Recalibrated Av. Absolute Mass Deviation [ppm] Mass Standard Deviation [ppm] Av. Absolute Mass Deviation [mDa] Mass Standard Deviation [mDa] Label free norm param 20210223_TTP_P_SILS5622_RA1_1_9559 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 13365 51270 0 33289 33289 0 0 2212 2212 0 0 6.6 6.6 0 0 1590 1818172 57488 3.2 49924 87 121244 33317 31051 27 35 30827 93 + 20210223_TTP_P_SILS5623_RA2_1_9560 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12052 52642 0 41238 41238 0 0 5571 5571 0 0 14 14 0 0 3469 2261316 76058 3.4 64020 84 154873 41254 38741 27 33 37453 91 + 20210223_TTP_P_SILS5624_RA3_1_9561 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 11831 52902 0 49841 49841 0 0 7034 7034 0 0 14 14 0 0 4180 3247062 99661 3.1 81078 81 210234 49853 46956 24 31 44149 89 + 20210223_TTP_P_SILS5625_RA4_1_9562 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12937 51249 0 46931 46931 0 0 6375 6375 0 0 14 14 0 0 3741 2822154 92032 3.3 77132 84 178387 46931 44648 26 32 42380 90 + 20210223_TTP_P_SILS5626_RA5_1_9563 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 13013 51215 0 45430 45430 0 0 8299 8299 0 0 18 18 0 0 4441 2336273 83636 3.6 72270 86 150250 45432 43663 30 34 41990 92 + 20210223_TTP_P_SILS5627_RA6_1_9564 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12877 51052 0 61852 61852 0 0 12461 12461 0 0 20 20 0 0 6544 3429162 110028 3.2 91126 83 240945 61857 59757 26 29 55334 89 + 20210223_TTP_P_SILS5628_RA7_1_9565 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12230 52008 0 54764 54764 0 0 12243 12243 0 0 22 22 0 0 6731 3008396 97147 3.2 81517 84 208960 54767 52991 26 30 49224 90 + 20210223_TTP_P_SILS5629_RA8_1_9566 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12374 52133 0 41348 41348 0 0 10023 10023 0 0 24 24 0 0 5358 2120957 74183 3.5 64157 86 140498 41351 40077 29 32 37979 92 + 20210223_TTP_P_SILS5630_RB1_1_9569 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12281 52805 0 40565 40565 0 0 9600 9600 0 0 24 24 0 0 5114 2183990 74767 3.4 64293 86 138047 40569 38791 29 31 37093 91 + 20210223_TTP_P_SILS5631_RB2_1_9570 WT1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 15160 48579 0 13395 13395 0 0 3855 3855 0 0 29 29 0 0 2198 893028 30956 3.5 27386 88 41198 13398 12694 33 35 12465 93 + 20210223_TTP_P_SILS5632_RB3_1_9571 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12130 52782 0 42225 42225 0 0 3010 3010 0 0 7.1 7.1 0 0 2032 2430456 74457 3.1 62829 84 164924 42241 39450 26 33 38407 91 + 20210223_TTP_P_SILS5633_RB4_1_9572 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 11760 52978 0 53481 53481 0 0 6797 6797 0 0 13 13 0 0 3948 3296591 98999 3 80423 81 231481 53502 50469 23 30 47237 88 + 20210223_TTP_P_SILS5634_RB5_1_9573 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12557 51877 0 49731 49731 0 0 7283 7283 0 0 15 15 0 0 4180 3144294 99533 3.2 81285 82 205914 49738 46915 24 31 44194 89 + 20210223_TTP_P_SILS5635_RB6_1_9574 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12622 51862 0 49722 49722 0 0 7941 7941 0 0 16 16 0 0 4384 2908141 97623 3.4 81550 84 183236 49724 47463 27 32 44935 90 + 20210223_TTP_P_SILS5636_RB7_1_9575 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12680 51699 0 49178 49178 0 0 9144 9144 0 0 19 19 0 0 4726 2646319 90226 3.4 77388 86 169037 49181 47294 29 32 45136 92 + 20210223_TTP_P_SILS5637_RB8_1_9576 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 13584 50234 0 61124 61124 0 0 12714 12714 0 0 21 21 0 0 6750 3265859 108651 3.3 90027 83 233744 61129 59019 26 29 54443 89 + 20210223_TTP_P_SILS5638_RC1_1_9580 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12087 53055 0 55100 55100 0 0 12236 12236 0 0 22 22 0 0 6621 2884078 96864 3.4 80951 84 208480 55105 53228 26 30 49377 90 + 20210223_TTP_P_SILS5639_RC2_1_9581 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12486 52086 0 42473 42473 0 0 9610 9610 0 0 23 23 0 0 4964 2086994 75218 3.6 64994 86 143269 42474 41137 30 32 38946 92 + 20210223_TTP_P_SILS5640_RC3_1_9582 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12286 51988 0 49383 49383 0 0 10640 10640 0 0 22 22 0 0 5589 2825793 91577 3.2 77191 84 184276 49383 46185 27 29 44361 90 + 20210223_TTP_P_SILS5641_RC4_1_9583 WT2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14255 49586 0 24780 24780 0 0 5663 5663 0 0 23 23 0 0 3229 1429994 49619 3.5 43052 87 80527 24782 22841 31 32 22776 92 + 20210223_TTP_P_SILS5642_RC5_1_9584 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12062 52774 0 42993 42993 0 0 3323 3323 0 0 7.7 7.7 0 0 2197 2497374 76204 3.1 64484 85 170203 43018 40136 25 33 39195 91 + 20210223_TTP_P_SILS5643_RC6_1_9585 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 11493 53422 0 54251 54251 0 0 6465 6465 0 0 12 12 0 0 3675 3375956 99946 3 81323 81 239836 54268 51136 23 31 48007 88 + 20210223_TTP_P_SILS5644_RC7_1_9586 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12190 52409 0 52174 52174 0 0 8679 8679 0 0 17 17 0 0 5125 3255555 103913 3.2 84336 81 214854 52182 49438 24 31 46157 88 + 20210223_TTP_P_SILS5645_RC8_1_9587 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12282 52288 0 48536 48536 0 0 7727 7727 0 0 16 16 0 0 4081 2721088 93893 3.5 79359 85 172270 48542 46269 28 33 44096 91 + 20210223_TTP_P_SILS5646_RD1_1_9590 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 13118 51745 0 50930 50930 0 0 9114 9114 0 0 18 18 0 0 4807 2704170 92682 3.4 79074 85 178108 50938 48966 29 32 46641 92 + 20210223_TTP_P_SILS5647_RD2_1_9591 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12725 52126 0 62027 62027 0 0 12797 12797 0 0 21 21 0 0 6549 3417536 111597 3.3 92639 83 238243 62028 59799 26 29 55544 90 + 20210223_TTP_P_SILS5648_RD3_1_9592 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12095 53067 0 57538 57538 0 0 12614 12614 0 0 22 22 0 0 6632 3021089 101324 3.4 84382 83 221762 57540 55569 26 29 51312 89 + 20210223_TTP_P_SILS5649_RD4_1_9593 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12538 52667 0 34486 34486 0 0 8011 8011 0 0 23 23 0 0 4110 1685341 62669 3.7 54883 88 110568 34492 33296 31 34 31899 92 + 20210223_TTP_P_SILS5650_RD5_1_9594 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 12331 52687 0 49844 49844 0 0 10890 10890 0 0 22 22 0 0 5658 2724321 92223 3.4 77632 84 182932 49844 46024 27 29 44695 90 + 20210223_TTP_P_SILS5651_RD6_1_9595 WT3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 13781 51083 0 17724 17724 0 0 4149 4149 0 0 23 23 0 0 2428 1150124 39495 3.4 34730 88 55298 17726 16103 32 34 16371 92 + 20210223_TTP_P_SILS5652_RD7_1_9596 M-CotER1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14769 49166 0 75425 75425 0 0 22311 22311 0 0 30 30 0 0 14844 4252516 121348 2.9 99659 82 320911 75427 72609 24 29 66904 89 + 20210223_TTP_P_SILS5653_RD8_1_9597 M-CotER2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14388 49277 0 73206 73206 0 0 23413 23413 0 0 32 32 0 0 15881 3897289 116812 3 96356 82 300197 73206 70483 24 30 65137 89 + 20210223_TTP_P_SILS5654_RE1_1_9601 M-CotER3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14336 49922 0 67455 67455 0 0 21305 21305 0 0 32 32 0 0 14878 3398260 105873 3.1 88386 83 264982 67455 65142 25 31 60384 90 + 20210223_TTP_P_SILS5655_RE2_1_9602 M-001R1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14279 49780 0 71278 71278 0 0 25403 25403 0 0 36 36 0 0 16808 3896711 115719 3 95367 82 287033 71280 68570 25 30 63393 89 + 20210223_TTP_P_SILS5656_RE3_1_9603 M-001R2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14571 49260 0 71720 71720 0 0 26258 26258 0 0 37 37 0 0 17200 3927775 116366 3 95900 82 291986 71723 68993 25 29 63871 89 + 20210223_TTP_P_SILS5657_RE4_1_9604 M-001R3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14471 49696 0 69570 69570 0 0 25125 25125 0 0 36 36 0 0 16882 3649518 112032 3.1 92573 83 279551 69572 66983 25 30 62048 89 + 20210223_TTP_P_SILS5658_RE5_1_9605 M-003R1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14750 49379 0 64693 64693 0 0 24766 24766 0 0 38 38 0 0 16785 3135960 103398 3.3 86221 83 245046 64694 62456 26 31 58024 90 + 20210223_TTP_P_SILS5659_RE6_1_9606 M-003R2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14157 50047 0 68914 68914 0 0 25157 25157 0 0 37 37 0 0 16966 3552660 111475 3.1 92557 83 270994 68916 66308 25 30 61651 89 + 20210223_TTP_P_SILS5660_RE7_1_9607 M-003R3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14572 49304 0 71047 71047 0 0 26212 26212 0 0 37 37 0 0 17393 3777057 115573 3.1 95313 82 285053 71048 68228 25 29 63354 89 + 20210223_TTP_P_SILS5661_RE8_1_9608 M-005R1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14624 49291 0 72094 72094 0 0 25390 25390 0 0 35 35 0 0 16923 3965217 118006 3 97231 82 296184 72096 69240 24 29 64082 89 + 20210223_TTP_P_SILS5662_GA1_1_9612 M-005R2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14492 49261 0 67291 67291 0 0 24094 24094 0 0 36 36 0 0 15245 3833812 111721 2.9 92801 83 271407 67293 64567 25 29 60333 90 + 20210223_TTP_P_SILS5663_GA2_1_9613 M-005R3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14607 49342 0 70176 70176 0 0 25232 25232 0 0 36 36 0 0 16924 3823643 113625 3 93662 82 290087 70179 67571 24 29 62396 89 + 20210223_TTP_P_SILS5664_GA3_1_9614 M-006R1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14434 49448 0 72237 72237 0 0 25588 25588 0 0 35 35 0 0 16910 4081940 117963 2.9 97086 82 302460 72238 69467 24 28 64336 89 + 20210223_TTP_P_SILS5665_GA4_1_9615 M-006R2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14527 49337 0 73572 73572 0 0 25578 25578 0 0 35 35 0 0 17080 4224336 120091 2.8 98717 82 314136 73574 70722 23 28 65470 89 + 20210223_TTP_P_SILS5666_GA5_1_9616 M-006R3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14684 49184 0 73217 73217 0 0 24736 24736 0 0 34 34 0 0 16747 4073087 118236 2.9 96987 82 309968 73218 70446 24 29 64879 89 + 20210223_TTP_P_SILS5667_GA6_1_9617 M-007R1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14054 50110 0 71962 71962 0 0 27798 27798 0 0 39 39 0 0 18506 3899946 116461 3 96109 83 296009 71964 69057 24 29 64037 89 + 20210223_TTP_P_SILS5668_GA7_1_9618 M-007R2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14551 49585 0 64650 64650 0 0 26762 26762 0 0 41 41 0 0 18236 3172971 102915 3.2 85943 84 250048 64654 62389 26 31 58018 90 + 20210223_TTP_P_SILS5669_GA8_1_9619 M-007R3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14199 49861 0 72162 72162 0 0 28223 28223 0 0 39 39 0 0 18728 3988306 117856 3 97072 82 299487 72165 69276 24 29 64227 89 + 20210223_TTP_P_SILS5670_GB1_1_9623 M-008R1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14617 49230 0 73115 73115 0 0 27981 27981 0 0 38 38 0 0 18680 3943287 118115 3 97124 82 303244 73117 70259 24 29 64947 89 + 20210223_TTP_P_SILS5671_GB2_1_9624 M-008R2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14520 49416 0 71579 71579 0 0 26890 26890 0 0 38 38 0 0 17737 3891360 116669 3 96174 82 293365 71584 68806 24 29 63663 89 + 20210223_TTP_P_SILS5672_GB3_1_9625 M-008R3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14446 49607 0 74545 74545 0 0 27692 27692 0 0 37 37 0 0 18485 4138774 120707 2.9 99069 82 316650 74548 71674 24 29 66098 89 + 20210223_TTP_P_SILS5673_GB4_1_9626 M-009R1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14527 49270 0 74207 74207 0 0 24160 24160 0 0 33 33 0 0 16153 4035099 119637 3 98289 82 309468 74209 71474 24 29 65927 89 + 20210223_TTP_P_SILS5674_GB5_1_9627 M-009R2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14525 49335 0 73883 73883 0 0 294 294 0 0 0.4 0.4 0 0 274 4047470 119364 2.9 98136 82 305387 73884 71076 24 29 65634 89 + 20210223_TTP_P_SILS5675_GB6_1_9628 M-009R3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14267 49973 0 70285 70285 0 0 23036 23036 0 0 33 33 0 0 15756 3656141 111651 3.1 91855 82 287537 70285 67986 24 30 62483 89 + 20210223_TTP_P_SILS5676_GB7_1_9629 Y-BR1 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14121 50141 0 73000 73000 0 0 15516 15516 0 0 21 21 0 0 10784 3764247 116234 3.1 96367 83 295558 73002 70433 25 30 65012 89 + 20210223_TTP_P_SILS5677_GB8_1_9630 Y-BR2 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14515 49402 0 77603 77603 0 0 17205 17205 0 0 22 22 0 0 11620 4169367 124171 3 101863 82 325776 77604 74654 24 29 68510 88 + 20210223_TTP_P_SILS5678_GC1_1_9633 Y-BR3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14622 49369 0 76375 76375 0 0 17151 17151 0 0 22 22 0 0 11751 3942557 120840 3.1 99320 82 314467 76377 73518 24 29 67529 88 + 20210223_TTP_P_SILS5679_GC2_1_9634 Y-DR3 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14559 49205 0 75398 75398 0 0 16637 16637 0 0 22 22 0 0 11594 3789269 118861 3.1 98023 82 303425 75398 72651 25 30 66909 89 + 20210223_TTP_P_SILS5680_GC3_1_9635 Y-DR4 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14608 49200 0 76587 76587 0 0 17500 17500 0 0 23 23 0 0 12074 3965655 121488 3.1 99910 82 312231 76589 73814 25 29 67879 89 + 20210223_TTP_P_SILS5681_GC4_1_9636 Y-DR5 Trypsin/P Specific False Oxidation (M) Carbamidomethyl (C) False False 1 2 TIMS-DDA 14015 50154 0 73626 73626 0 0 16841 16841 0 0 23 23 0 0 11741 3679981 115928 3.2 96234 83 292650 73628 70955 25 30 65549 89 + Total 812989 3043822 0 3537225 3537225 0 0 930734 930734 0 0 26 26 0 0 41831 193165794 14008895 3537493 3389913 25 30 3165307 89 0 0 0 0