MassIVE MSV000079962

Imported Reanalysis Dataset Public PXD003037

Systemic remodeling of translation efficiencies on oxygen stimulus.

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Description

Protein concentrations evolve under greater evolutionary constraint than mRNA levels. In addition, translation efficiency of mRNA populations represents the chief determinant of basal protein concentrations. This raises a fundamental question as to how mRNA and protein levels are actively coordinated in dynamic systems responding to acute physiological stimuli. This report examines the contributions of mRNA abundance and translation efficiency to protein output in cells responding to oxygen stimulus. We show that alternative translation efficiencies, not mRNA levels, represent the major adaptive mechanism that governs the cellular response to perturbations in [O2]. This phenomenon is coordinated by eIF4F under atmospheric [O2] and the previously uncharacterized hypoxic eIF4F (eIF4FH) under low [O2]. The oxygen-regulated remodeling of translation efficiency enables oxygenated and hypoxic cells to produce surprisingly divergent translatomes of similar complexity, with minimal dependence on mRNA levels. Changes in mRNA concentrations observed between normoxic and hypoxic cells are likely neutral, as they are during evolution. We propose that mRNAs contain hard-wired translation efficiency determinants for their triage by the translation apparatus on [O2] stimulus. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Hypoxia ; cancer ; translation ; RNA sequencing ; SILAC ; eIF4E ; eIF4E2

Contact

Principal Investigators:
(in alphabetical order) 		Dr Stephen Lee
Submitting User: ccms

Publications

Ho JJ, Wang M, Audas TE, Kwon D, Carlsson SK, Timpano S, Evagelou SL, Brothers S, Gonzalgo ML, Krieger JR, Chen S, Uniacke J, Lee S.
Systemic Reprogramming of Translation Efficiencies on Oxygen Stimulus.
Cell Rep. 2016 Feb 16;14(6):1293-300. Epub 2016 Feb 4.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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