We used a state of the art proteomics workflow, based upon nano-UPLC-MS\MS and advanced database searching to identify oxidative modifications to functional residues of hemoglobin subunit beta . Progressive accumulation of oxidized residues in stored erythrocytes and selective removal in vesicles was observed, further substantiating the hypothesis about vesiculation as a self-protecting mechanism in aging erthrocytes.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: RBC Transfusion ; LC-MSMS ; Hemoglobin ; Oxidation
Principal Investigators: (in alphabetical order) |
Dr Angelo D'Alessandro |
Submitting User: | ccms |
Wither M, Dzieciatkowska M, Nemkov T, Strop P, D'Alessandro A, Hansen KC.
Hemoglobin oxidation at functional amino acid residues during routine storage of red blood cells.
Transfusion. 2016 Feb;56(2):421-6. Epub 2015 Oct 1.
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