MassIVE MSV000086844

Complete Public PXD024262

Resource allocation in the archaeon Methanococcus maripaludis during slow growth

Description

We conducted a systems-level study on the physiology of slow growth in the archaeon Methanococcus maripaludis revealing a strategy for acclimation to slow growth that relies on redistribution of energy for cellular maintenance and changes in catabolic and ribosomal activity rather than gene regulation, a strategy that is distinct from well-known heterotrophic model bacteria such as E. coli. This dataset describes the proteome response of Methanococcus maripaludis to a formate (energy) limitation when grown in a chemostat at different growth rates. Samples contain both 14N and 15N-labeled peptides, with the 15N reference generated as a combination of batch exponential culture and a stationary phase cultures. [doi:10.25345/C51N58] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: archaea ; formate ; methanococcus maripaludis ; methanogen ; N15

Contact

Principal Investigators:
(in alphabetical order)
Alfred M. Spormann, Stanford University, United States
James R. Williamson, The Scripps Research Institute, United States
Submitting User: patsalo

Publications

Müller AL, Gu W, Patsalo V, Deutzmann JS, Williamson JR, Spormann AM.
An alternative resource allocation strategy in the chemolithoautotrophic archaeon Methanococcus maripaludis.
Proc Natl Acad Sci U S A.

Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
Browse Dataset Files Browse Results
 
FTP Download Link (click to copy):

- Dataset Reanalyses


+ Dataset History


Click here to queue conversion of this dataset's submitted spectrum files to open formats (e.g. mzML). This process may take some time.

When complete, the converted files will be available in the "ccms_peak" subdirectory of the dataset's FTP space (accessible via the "FTP Download" link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.