Here we present a strategy for the identification and quantitation of histone methylation that utilizes nondeuterated acetic anhydride to fully acetylate all free lysine residues prior to digestion with trypsin and analysis by LC-MS/MS.
[doi:10.25345/C5QJ3P]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Methylation ; Histone ; Acetylation
Principal Investigators: (in alphabetical order) |
Roland S. Annan, GlaxoSmithKline, Inc., USA |
Submitting User: | cwagner68 |
Zappacosta F, Wagner CD, Della Pietra A, Gerhart SV, Keenan K, Korenchuck S, Quinn CJ, Barbash O, McCabe MT, Annan RS.
A Chemical Acetylation-Based Mass Spectrometry Platform for Histone Methylation Profiling.
Mol Cell Proteomics. Epub 2021 Mar 26.
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