Peptide features were identified with the Progenesis algorithm (v4.0.6403), aligned across all files, and annotated with the peptide identification resulting from the database search result from Comet. Unique semi-tryptic peptides corresponding to the spiked-in proteins were quantified using the feature areas as calculated by the Progenesis algorithm. The quantification result is the same as in RMSV000000249.3. The differential abundance analysis was performed by MSstats (v3.16.2) R package: Top 10 features were used for this reanalysis. Details for data processing and statistical analysis are available in description.pdf ('Methods' folder).
**Publication : Tsai TH, Choi M, Banfai B, Liu Y, MacLean BX, Dunkley T, Vitek O. (2020). Selection of features with consistent proles improves relative protein quantification in mass spectrometry experiments. Molecular & Cellular Proteomics. 2020 March 31, 19(6) 944-959, doi:10.1074/mcp.RA119.001792. PMID: 32234965.
[doi:10.25345/C5TT7W]
[See
results attachment job
for details]
Keywords: MassIVE.quant reviewed - Platinum
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Quantification Results | |
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Browse Reanalysis Files | |
Browse Quantification Results | Browse Metadata |
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