MassIVE MSV000083640

Partial Public PXD013293

Rapid discovery of drug target engagement by isothermal shift assay

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Description

Determining protein targets directly bound by drug molecules remains challenging. Here we present the isothermal shift assay, iTSA, for rapid unbiased identification of drug targets. Compared with thermal proteome profiling, the prevailing method for target engagement, iTSA offers a simplified workflow, 4- fold higher throughput, and a multiplexed experimental design with higher replication. We demonstrate its application to determination of target engagement for several kinase inhibitors in lysates and living cells. [doi:10.25345/C55036] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: iTSA ; proteome ; thermal shift

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Principal Investigators:
(in alphabetical order) 		William Old, University of Colorado Boulder, United States
Submitting User: wold_cub
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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