MassIVE MSV000092784

Partial Public PXD044943

In vitro phosphorylation of trypsin-digested K562 cell lysate by TAM family kinases

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Description

K562 cell lysate was digested with trypsin, dephosphorylated with lambda phosphatase, and treated in an in vitro kinase reaction with Axl, Mer or Tyro3 kinase, alongside a control reaction for each that contained no kinase. Samples were phosphoenriched with the SMOAC approach (TiO2 and FeNTA) and analyzed on an Orbitrap-Velos. Peptides were identified using PEAKS Studio X Pro. [doi:10.25345/C5513V612] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: kinase ; phosphopeptide ; AXL ; MERTK ; TYRO3 ; KALIP

Contact

Principal Investigators:
(in alphabetical order) 		Laurie Parker, University of Minnesota, USA
Submitting User: llparker

Publications

Widstrom NE, Andrianov GV, Heier JL, Heier C, Karanicolas J, Parker LL.
Novel Substrate Prediction for the TAM Family of RTKs Using Phosphoproteomics and Structure-Based Modeling.
ACS Chem Biol. 2024 Jan 19;19(1):117-128. Epub 2023 Dec 30.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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