Description
The plasmids were then transiently transfected into HEK293F expression system for overexpression. After incubation for 48 h, the cells were collected, washed one with ice-cold PBS. The resuspended cells were then lysed by sonication, and the lysate supernatant after centrifugation was incubated with M2-FLAG affinity beads for 1.5 h. After several round of washing with buffer B, the FLAG-affinity proteins were eluted by 0.5 mg/mL FLAG peptide dissolved in buffer C. The elute was further purified by gel filtration (Superose 6 Increase Columns, Cytiva) using buffer C. The fraction at 0.5 mL/tube was collected starting from 7 mL. Concentrate a few tubes from the main peak diagram. The purified FLAG-BIRC6 complex was analyzed by mass spectrometry following separation of the complex by SDS-PAGE. The full lane was sliced into 6 pieces from top to bottom with assigned sample numbers: 1, 2-1, 2-2, 3-1, 3-2, and 4.
[doi:10.25345/C52J68F40]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: BIRC6 ; UBA6 ; Smac
Contact
Principal Investigators:
(in alphabetical order)
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Xiao-Bo Qiu, Beijing Normal University, China
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| Submitting User: |
Liuss
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Conditions:
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Biological Replicates:
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Technical Replicates:
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| Identification Results |
Proteins (Human, Remapped):
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Proteins (Reported):
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Peptides:
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Variant Peptides:
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PSMs:
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Differential Proteins:
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Quantified Proteins:
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Number of distinct conditions across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct condition labels are counted across all files submitted in the "Metadata" category
having a "Condition" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct replicate labels are counted across all files submitted in the "Metadata" category
having a "BioReplicate" or "Replicate" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
associated with this dataset.
The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
considered.
"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.