The Kidney Precision Medicine Project (KPMP) is funded by the NIDDK for "the purpose of understanding and finding new ways to treat chronic kidney disease and acute kidney injury. " To accomplish this goal, the KPMP chose several technologies to successfully deliver their goals. One of the technologies chosen was using a combination of laser capture microdissection (LCM) in combination with LC -MS /MS to characterize quantitative protein changes in the diseased kidney. The data presented here is from the initial pilot study using normal kidney biopsies for proof of principal. Samples analyzed were either LCM collected glomeruli or tubulointerstitium. Protein was retrieved from the LCM collected tissue, proteins were trypsinized and samples run on the fusion orbitrap.
[doi:10.25345/C5VT1GT5W]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Laser Capture Microdissection ; Normal Kidney Glomeruli ; Normal Kidney Tubulointerstitium ; Spectral Counts ; Thermo Orbitrap Eclipse ; Bottom-up Proteomics
Principal Investigators: (in alphabetical order) |
Samir Parikh, The Ohio State University Wexner Medical Center, United States |
Submitting User: | gardner207 |
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