This work describes a micro-scale basic reverse phase liquid chromatographic (bRPLC) fractionation method that offers high reproducibility and efficiency for peptide mixtures from small (5-20 g) samples. Shotgun and targeted proteomic analysis were performed to detect differentially expressed proteins from lung tumor cell lines that are sensitive (11-18) and resistant (11-18R) to the tyrosine kinase inhibitor erlotinib using micro-bRPLC.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: cell lines ; colon tumor tissue
Principal Investigators: (in alphabetical order) |
Daniel Liebler |
Submitting User: | rslebos |
Lee HJ, Kim HJ, Liebler DC.
Efficient Microscale Basic Reverse Phase Peptide Fractionation for Global and Targeted Proteomics.
J. Proteome Res. 2016 Jul 1;15(7):2346-54. Epub 2016 Jun 13.
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