Numerous RNAs exhibit specific distribution patterns in mammalian cells. However, the
functional and mechanistic consequences are relatively unknown. We investigate here the
functional role of RNA localization at cellular protrusions of mesenchymal migrating cells, using
as a model the RAB13 RNA, which encodes a GTPase important for vesicle-mediated
membrane trafficking. While RAB13 RNA is enriched at peripheral protrusions, the expressed
protein is concentrated perinuclearly. By specifically preventing RAB13 RNA localization, we
show that peripheral RAB13 translation is not important for the overall distribution of the RAB13
protein, or its ability to associate with membranes, but is required for full activation of the
GTPase and for efficient cell migration. This effect is mediated by a co-translational association
of RAB13 with the exchange factor RABIF. Our results indicate that RAB13-RABIF association
at the periphery is required for directing RAB13 GTPase activity to promote cell migration. Thus,
translation of RAB13 in specific subcellular environments imparts the protein with distinct
properties and highlights a means of controlling protein function through local RNA translation.
[doi:10.25345/C51B3D]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Rab13 ; Rab interactome
Principal Investigators: (in alphabetical order) |
Lisa Jenkins, NCI, NIH, United States |
Submitting User: | lisamiljenk |
Moissoglu K, Stueland M, Gasparski AN, Wang T, Jenkins LM, Hastings ML, Mili S.
RNA localization and co-translational interactions control RAB13 GTPase function and cell migration.
EMBO J. 2020 Nov 2;39(21):e104958. Epub 2020 Sep 18.
Number of Files: | |
Total Size: | |
Spectra: | |
Subscribers: | |
Owner | Reanalyses | |
---|---|---|
Experimental Design | ||
Conditions:
|
||
Biological Replicates:
|
||
Technical Replicates:
|
||
Identification Results | ||
Proteins (Human, Remapped):
|
||
Proteins (Reported):
|
||
Peptides:
|
||
Variant Peptides:
|
||
PSMs:
|
||
Quantification Results | ||
Differential Proteins:
|
||
Quantified Proteins:
|
||
Browse Dataset Files | Browse Results |
FTP Download Link (click to copy):
|