MassIVE MSV000092040

Partial Public PXD042513

Combined transcriptomics and proteomics analysis unveiled the impact of vitamin C in modulating specific mitochondrial protein abundance in the mouse liver

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Description

: In the present study, we used the Gulo-/- female and male mice model which depends entirely on ascorbate derived from the diet. We tested different concentrations of ascorbate ranging from 0 to 0.4% (w/v) ascorbate, added into drinking water, until the age of four months. Importantly, the levels of ascorbate found in the liver of Gulo-/- mice reflect the amounts of ascorbate provided in drinking water. We performed label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) global quantitative proteomic profiling to identify and quantify proteins in whole liver extracts from our different ascorbate treated cohorts of Gulo-/- females and males. We compared the proteomic profiles to the transcriptomic profiles of the same treated animals. Although several proteins of the mitochondrial complex III significantly correlated with vitamin C concentrations, their corresponding transcripts did not correlate with vitamin C in both females and males. Such observations were confirmed by western blot and quantitative RT-PCR analyses, respectively. Our findings suggest that transcriptome profiling alone provides an incomplete picture of molecular changes associated with vitamin C deficiency in the liver of mice and examination of changes in protein abundances is essential to unveil variations that are not transcriptionally regulated. [doi:10.25345/C5PV6BH7V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Vitamin C, gulonolactone oxidase, mass spectrometry, mitochondrial complex III, transcription, liver, mouse

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Principal Investigators:
(in alphabetical order) 		Michel Lebel, Centre Hospitalier Universite Laval, Canada
Submitting User: MicLeb
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