MassIVE MSV000083502

Partial Public PXD012916

Analysis of proteolytic processes in plasma and bone marrow interstitial fluid of pediatric B-ALL patients by HUNTER (High-efficiency Undecanal-based N Termini EnRichment)

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Description

Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samples. Automated enrichment and identification of distinct N-terminal profiles in blood and bone marrow plasma from pediatric cancer as one of the applications of HUNTER. [doi:10.25345/C5V90C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: HUNTER ; N termini ; proteolytic proteoforms ; B-ALL ; plasma ; pediatric

Contact

Principal Investigators:
(in alphabetical order) 		Dr. Philipp Lange, The University of British Columbia, Canada
Dr. Pitter F. Huesgen, University of Cologne, Germany
Submitting User: LangeLab

Publications

Weng SHS, Demir F, Ergin EK, Dirnberger S, Uzozie A, Tuscher D, Nierves L, Tsui J, Huesgen PF, Lange PF.
Sensitive determination of proteolytic proteoforms in limited microscale proteome samples.
Mol. Cell Proteomics. Epub 2019 Aug 30.

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