MassIVE MSV000083838

Partial Public PXD013974

Hypoxia-induced reprogramming of the cardiac phenotype in American alligators (Alligator mississippiensis) revealed by quantitative proteomics

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Description

Hypoxic exposure during development can have a profound influence on offspring physiology, including cardiac dysfunction, yet many reptile embryos naturally experience periods of hypoxia in buried nests. American alligators experimentally exposed to developmental hypoxia demonstrate morphological and functional changes to the heart that persist into later life stages; however, the molecular bases of these changes remain unknown. We tested if targeted and persistent changes in steady-state protein expression underlie this hypoxic heart phenotype, using isobaric tags for relative and absolute quantitation (iTRAQ) proteomics. Alligator eggs were reared under normoxia or 10% hypoxia, then either sampled (embryo) or returned to normoxia for 2 years (juvenile). Three salient findings emerge from the integrated analysis of the 145 differentially expressed proteins in hypoxia-reared animals: (1) significant protein-protein interaction networks were identified only in up-regulated, indicating that the effects of developmental hypoxia are stimulatory and directed; (2) the up-regulated proteins substantially enriched processes related to protein turnover, cellular organization, and metabolic pathways, supporting increased resource allocation towards building and maintaining a higher functioning heart; and (3) the juvenile cardiac proteome retained many of the signature changes observed in embryonic hearts, supporting long-term reprogramming of cardiac myocytes induced by hypoxia during critical periods of development. [doi:10.25345/C55W5X] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: heart ; reptile ; oxygen ; iTRAQ ; developmental programming ; phenotypic plasticity

Contact

Principal Investigators:
(in alphabetical order) 		Sarah Alderman, University of Guelph, Canada
Submitting User: alderman

Publications

Alderman SL, Crossley DA, Elsey RM, Gillis TE.
Hypoxia-induced reprogramming of the cardiac phenotype in American alligators (Alligator mississippiensis) revealed by quantitative proteomics.
Sci Rep. 2019 Jun 13;9(1):8592. Epub 2019 Jun 13.

Alderman SL, Crossley DA, Elsey RM, Gillis TE.
Growing up gator: a proteomic perspective on cardiac maturation in an oviparous reptile, the American alligator (Alligator mississippiensis).
J Comp Physiol B. 2020 Mar;190(2):243-252. Epub 2020 Jan 22.

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