MassIVE MSV000084654

Partial Public PXD016558

EGFR inhibition triggers an adaptive response by co-opting antiviral signaling pathways in lung cancer

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Description

HCC827 cells were treated with 0.1 microM erlotinib for 0, 2, 6, and 24 hours. Cell lysates were immunoprecipitated with TBK1 antibody. Antibody enriched protein samples were run on SDS-PAGE gels and submitted to UT Southwestern Proteomics Core Facility for Mass Spectrometry. Protein gel pieces were digested overnight with trypsin (Pierce) following reduction and alkylation with DTT and iodoacetamide (Sigma Aldrich. The samples then underwent solid-phase extraction cleanup with Oasis HLB microelution plates (Waters) and the resulting samples were analyzed by LC-MS-MS, using an Orbitrap Fusion Lumos mass spectrometer (Thermo Electron) coupled to an Ultimate 3000 RSLC-Nano liquid chromatography systems (Dionex). [doi:10.25345/C5RT0M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: EGFR inhibition, therapeutic resistance, Type I interferons, TBK1, IRF3,RIG-I, lung cancer, NSCLC, erlotinib, EGFR wild type, Anifrolumab

Contact

Principal Investigators:
(in alphabetical order) 		Amyn A. Habib, University of Texas Southwestern Medical Center, North Texas VA Healthcare System, Mail Code 151, 4500 South Lancaster Road, Dallas TX 75216, United States
Submitting User: mogoodarzi
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