MassIVE MSV000089569

Partial Public PXD034206

Simultaneous extraction of calcium phosphates and proteins from fish bones. Innovative valorisation of food by-products.

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Description

By products of the fish industry can be a source of valuable molecules, with different technological applications. Fish bones, in particular, contain both organic and inorganic phases (collagen, other proteins and calcium phosphate respectively), with uses in cosmetics and biomedicine. Using the most common extraction protocols, only of the two phases can be extracted, while the other one is degraded. Investigation of the organic phase composition by proteomic related procedures followed by LC MS MS analysis, showed that the main extracted proteins were collagen in its different forms (especially alpha 1); some differences were observed between salmon and sea bream and between the methods of extraction. Biocompatibility tests performed on both organic and inorganic extracts (with fibroblast and osteoblast like cellular lines) showed their non toxicity and, hence, their suitability for cosmetic or biomedical applications. [doi:10.25345/C5D21RP0C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: LC-MS/MS, fish bone proteins

Contact

Principal Investigators:
(in alphabetical order) 		Serena Camerini, Istituto superiore di sanita, Italy
serena camerini, istituto superiore sanita, italy
Submitting User: serena_camerini

Publications

Alessio Adamiano Stefania Scialla Francesca Carella Marialuisa Casella Serena Camerini Alessandra Quarta Alexandra Muntiu Francesca Ferrari Alberto Vitali Michele Iafisco Clara Piccirillo.
Simultaneous extraction of calcium phosphates and proteins from fish bones. Innovative valorisation of food by-products.
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.