MassIVE MSV000096868

Partial Public PXD059871

Quantitative proteomic analysis reveals JMJD6 and DNAJB11 as endogenous substrates of E3 ligase RFFL

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Description

Immunoprecipitation experiments using anti-RFFL antibody. Samples were loaded in technical duplicates in EvoTips, and chromatographic separation was done on an in-house column and the 30 samples per day (SPD) method on Evosep One system (Evosep). Peptides were detected and fragmented using timsTOF Pro 2 mass spectrometer (Bruker Daltonics) in data-dependent mode with parallel accumulation serial fragmentation (PASEF) enabled. The raw data was analyzed using Fragpipe (v 22.0). MS/MS spectra were searched using MSFragger (v 4.1) against forward and reversed Homo sapiens UniProt sequence database (UP000005640) supplemented with common contaminants (total 20,979 sequences excluding decoys). [doi:10.25345/C5TT4G54Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: RFFL ; Interactome ; E3 ligase ; Substrates ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Prof. S. Murty Srinivasula, Indian Institute of Science Education and Research Thiruvananthapuram, India
Submitting User: Narendradev_N

Publications

Narendradev ND, Marathe S, Baboo S, McClatchy DB, Diedrich JK, Jain P, Purwar R, Yates JR, Srinivasula SM.
Quantitative Proteomic Analysis Reveals JMJD6 and DNAJB11 as Endogenous Substrates of E3 Ligase RFFL.
J Proteome Res. Epub 2025 Jun 26.

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