MassIVE MSV000081885

Partial Public PXD008597

Genus wide venom proteomics of cobras (Naja)

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Description

Genus-wide proteomics analysis of cobra (Naja) venoms. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive) . Spectrum-Protein matching was performed with TopPic1.1 against a genus wide translated transcriptome database and NCBI protein entries from the najas. For bottom-up analysis, HPLC separated fraction of venom were dried down, reduced with triethylphosphine and alkylated with 2-iodoethanol. HPLC-MS/MS experiments were performed on normalflow UHPLC-QTOF system (AB Sciex 5600 TripleTOF). Data analysis (PSM) was performed ProteinPilot version 4.0 and the transcriptome derived protein sequence database. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Top-down Proteomics ; Top-down Venomics ; Naja ; Snake venom ; Venom Proteomics ; Cobra

Contact

Principal Investigators:
(in alphabetical order) 		Daniel Petras, University of California San Diego, USA
Eivind Undheim, The University of Queensland, Australia
Submitting User: daniel

Publications

Kazandjian TD, Petras D, Robinson SD, van Thiel J, Greene HW, Arbuckle K, Barlow A, Carter DA, Wouters RM, Whiteley G, Wagstaff SC, Arias AS, Albulescu LO, Plettenberg Laing A, Hall C, Heap A, Penrhyn-Lowe S, McCabe CV, Ainsworth S, da Silva RR, Dorrestein PC, Richardson MK, Gutiérrez JM, Calvete JJ, Harrison RA, Vetter I, Undheim EAB, Wüster W, Casewell NR.
Convergent evolution of pain-inducing defensive venom components in spitting cobras.
Science. 2021 Jan 22;371(6527):386-390.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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