MassIVE MSV000090559

Complete Public PXD037611

Ammonium Sulfate-based Prefractionation Improved Carbonylated Proteome Coverage in Arabidopsis thaliana Leaf Extract

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Description

The hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed to improve the detection of the carbonylated proteins in leaf extract. A total of 122 carbonylated proteins were identified, of which 77 were found in the ammonium sulfate fractions only. This prefractionation method allowed to identify 63% more carbonylated proteins compared to the number of carbonylated proteins identified from the total crude extract without prefractionation. Our results thus showed that a simple offline prefractionation of the proteome by ammonium sulfate prior to liquid chromatography-tandem mass spectrometry significantly improved the detection of carbonylated proteins in Arabidopsis leaf extract. [doi:10.25345/C5SB3X374] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Aldehyde reactive probe ; peptide level enrichment ; protein carbonylation ; proteome offline prefractionation

Contact

Principal Investigators:
(in alphabetical order) 		Tagnon Missihoun, University of Quebec Trois-Rivieres, Canada
Submitting User: Tagnon

Publications

Tola AJ, Missihoun TD.
Ammonium sulfate-based prefractionation improved proteome coverage and detection of carbonylated proteins in Arabidopsis thaliana leaf extract.
Planta. 2023 Feb 19;257(3):62. Epub 2023 Mar 19.

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