Venous blood was collected in Li-heparin or EDTA blood tubes and loaded onto 20 uL Mitra devices or separated to plasma as detailed in metadata. Samples were denatured in 5.5% SDC, reduced and alkylated, and digested with TPCK-trypsin followed by acid precipitation. 1 mg of filtered precipitates were enriched for phosphopeptides using TiO. Data was acquied using nanoLC, Evosep One or SPE-ZipChip. Data was acquired by DIA (Exploris 480 or TimsTOF Pro 2) or LFQ-DDA. DIA data was analyzed with Spectronaut 18 and DDA with Skyline.
[doi:10.25345/C5028PQ5B]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: phosphorylation ; data-independent acquisition
Principal Investigators: (in alphabetical order) |
Matthew W. Foster, Duke University, USA |
Submitting User: | mwfoster |
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