RRS1 was fused with highly active biotin ligase TurboID tagged with a FLAG epitope for proximity labeling.Biotin treatment of transgenic seedlings induced the overall biotinylation output signal.The total biotinylated proteins were enriched using streptavidin-conjugated beads and analyzed via liquid chromatography/tandem mass spectrometry analysis.
[doi:10.25345/C53R0Q56V]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: RRS1-R ; Arabidopsis ; TurboID ; DatasetType:Proteomics
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Hailong Guo, China Agricultural University, China |
| Submitting User: | zhiyichen |
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