MassIVE MSV000098983

Partial Public PXD067898

Li et al., Xylosyltransferase Bump-and-hole Engineering to Chemically Manipulate Proteoglycans in Mammalian Cells: CHO-pgs745 cells with 50 uM compound 1

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Description

CHO pgs-745 cells transfected with plasmids encoding for WT or bump-and-hole-engineered human XYLT1 or XYLT2 were fed with 6AzGlc-1-phosphate precursor (50 uM) or DMSO and secretome collected. Glycoproteins were clicked with acid-cleavable biotin-alkyne, enriched on neutravidin and on-bead digested with trypsin. Peptides were desalted and run on TIMS-ToF Pro. [doi:10.25345/C5R786243] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Glycosaminoglycan, chemical proteomics, glycosylation, glycosyltransferase ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Benjamin Schumann, TUD Dresden University of Technology, Germany
Submitting User: bschumann
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