To define the mitochondrial complexes interacting with MICU proteins we evaluated the mtCU-independent interactome of MICU1 in a co-expression system with MICU2 and/or MICU3. MICU1-containing MICU permutation plasmids (MICU1-HA, MICU1-HA-T2a-MICU2-Myc, MICU1-HA-T2a-MICU3-Flag, MICU1-HA-T2a-MICU2-Myc-T2a-MICU3-Flag) were expressed in Mcu-/- HEK 293T cells. IPs of MICU1-HA were performed and prepped for liquid chromatography mass spec (LC/MS) to identify interacting proteins
[doi:10.25345/C5XW4878P]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Elrod ; MICU1 ; MICU2 ; MICU3 ; mitochondria ; DatasetType:Proteomics
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Principal Investigators: (in alphabetical order) |
John Elrod, Lewis Katz School of Medicine at Temple University, USA |
| Submitting User: | HenryCohen1996 |
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