MassIVE MSV000087154

Partial Public PXD025157

High resolution time-lapse proteomic analysis of sporulation

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Description

Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome changeover during sporulation, a time-lapse proteomic analysis was performed in a cell population that was induced to sporulate synchronously. Here, we are the first to comprehensively investigate the changeover of sporulation regulatory proteins, coat proteins and other proteins involved in sporulation and spore biogenesis. Protein co-expression analysis revealed four co-expressed modules. Modules brown and green are upregulated during sporulation and are annotated as associated with sporulation. Module blue, which is negatively correlated with modules brown and green, comprised ribosomal and metabolic proteins, and module yellow is co-expressed with module blue and modules brown & green. Notably, several proteins not belonging to any of the known transcription regulons were identified as co-expressed with modules brown and green. We speculate that they may also play roles during sporulation. Finally, amounts of some coat proteins, for example morphogenetic coat proteins, decreased late in sporulation. While we speculate on their possible role in guiding or helping assembly of other coat proteins, after which they can be disposed of, such a hypothesis remains to be experimentally addressed. [doi:10.25345/C5NV4J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Bacillus subtilis ; Sporulation ; quantitative mass spectrometry ; proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Gertjan Kramer, University of Amsterdam, Netherlands
Submitting User: gkramer

Publications

Zhiwei Tu, Henk L Dekker, Winfried Roseboom, Bhagyashree N Swarge, Peter Setlow, Stanley Brul, Gertjan Kramer.
High Resolution Analysis of Proteome Dynamics during Bacillus subtilis Sporulation.
Int J Mol Sci . 2021 Aug 28;22(17):9345.

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