Label free mass spectrometry analysis to identify potential effectors of the ARL5A and ARL5B GTPases. During the MitoID proximity biotinylation experiment, bait constructs were made by fusing sequences encoding a mitochondrial-targeting sequence (MTS) from the outer mitochondrial membrane protein TOM20, along with the biotin ligase BioID2, to the N-terminus of constitutively active (Q70L) or inactive (T30N) forms of human ARL5A or ARL5B lacking the N-terminal amphipathic helix. MTS-BioID2 was used as a negative control.
[doi:10.25345/C56688W3W]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Mass spectrometry ; label-free quantitation
Principal Investigators: (in alphabetical order) |
Juan Bonifacino, National Institute of Child Health and Human Development, National Institutes of Health, USA |
Submitting User: | psfuserdata |
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