MassIVE MSV000084506

Partial Public PXD016028

Exoprotoemics of pseudomonas putida KT2440 outer membrane vesicles (OMV) and vesicle-free secretome (VSF)

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Description

Lignin is an abundant and recalcitrant component of plant cell walls. While lignin degradation in Nature is typically attributed to fungi, growing evidence suggests that bacteria can also modify this complex biopolymer. However, the spatiotemporal mechanisms for this modification remain unclear. Improved understanding of this biological and extracellular process would aid in our collective knowledge of both carbon cycling and advanced microbial strategies to valorize lignin. In this data set, we examined the exoproteome of Pseudomonas putida KT2440 grown with on lignin-rich media to characterize the secretion of abundant outer membrane vesicles (OMVs). Proteomic analysis revealed that the exoproteome is 20% more sub-compartmentalized into OMVs and OMV protein cargo are 52% more dynamic in lignin-rich media as compared to lignin-free media. [doi:10.25345/C5467G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: exoproteomics ; proteomics ; LC-MS/MS ; outer membrane vesicles

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Principal Investigators:
(in alphabetical order) 		Gregg Beckham, National Bioenergy Center, National Renewable Energy Laboratory, USA
Submitting User: pabraham_ORNL
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