Ascrobate peroxidase (APEX) was expressed as a fusion protein and directed to primary cilia of mouse inner medullary collecting duct (mIMCD-3) cells, where it was used for proximity labeling to biotinylate proteins, which are subsequently enriched by streptavidin chromatography and identified by mass spectrometry. Cilia-APEX proteomics profiling compared the cilia proteomes of wild-type and Arl6-/- cilia (generated by CRISPR/Cas9-mediated genome editing) by spectral counting.
[doi:10.25345/C5707WT0V]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Proximity Labeling Proteomics ; cilia-APEX profiling
Principal Investigators: (in alphabetical order) |
Maxence V. Nachury, University of California San Francisco, USA |
Submitting User: | MickLab |
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