Gene editing was used to modify an endogenous allele of USP13 in human HCT116 cells with the addition of a 1X FLAG/Streptavidin Binding Peptide dual epitope tag. Nuclear lysates were prepared and dual affinity purification was performed. Then, GelC/MS/MS was performed to identify proteins that were present after dual affinity purification from dual epitope-tagged cells but not from dual affinity purification from untagged parental cells.
[doi:10.25345/C5XB6M]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: deubiquitination, chromatin, cell cycle, cohesin, USP13
Principal Investigators: (in alphabetical order) |
Todd Waldman, Georgetown University School of Medicine, USA |
Submitting User: | bbudnik |
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