MassIVE MSV000099026

Partial Public PXD068054

Analyzes of PEGylation of a Heterogeneous Therapeutic Protein by Direct Mass Technology and Data Dependent Acquisition

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Description

Intact mass of a heterogeneous therapeutic protein was determined by Direct Mass Technology (Orbitrap based charge detection mass spectrometry), proving mono PEGylation of the biologic. Furthermore, using multiple enzyme combinations, major PEGylation sites of the protein were mapped by finetuning instrument parameters and chromatographic conditions. [doi:10.25345/C5639KJ5F] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Charge Detection Mass Spectrometry ; Data Dependent Acquisition ; PEGylation Mapping ; DatasetType:Other (Characterization)

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Principal Investigators:
(in alphabetical order) 		Zoltan Szabo, Thermo Fisher Scientific, United States
Submitting User: ZoltanSzabo1972
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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