We demonstrated high-resolution tandem mass spectrometry with an ultra-high-performance liquid chromatography for characterization and comparison between biologics and biosimilar of infliximab at an advanced level. Comparing the N- and O-glycopeptides profiles, a total of 49 and 54 glycopeptides was identified for each product of the biologics and biosimilar, respectively. We also discovered one novel N-glycosylation site at the light chain from both biopharmaceuticals and one novel O-glycopeptide at the heavy chain from only biosimilar. Site-specific glycopeptide analysis process will be a robust and useful technique for evaluating therapeutic mAbs and complex glycoprotein products.
[doi:10.25345/C5154DT0S]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: glycoproteome ; infliximab ; monoclonal antibody ; biopharmaceuticals
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Heeyoun Hwang, Korea Basic Science Institute, Rep of Korea |
Submitting User: | tilldawn |
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