MassIVE MSV000097047

Partial Public PXD060511

Novel antimicrobial peptides and peptide-microbiome crosstalk in Appalachian salamander skin

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Description

Host antimicrobial peptides (AMPs) are an ancient defense system found in all multicellular organisms that interact with host microbiomes. We used multi-omics and mathematical tools to discover new AMPs and examine AMP-microbial interactions in three Appalachian salamander species (Plethodon cinereus, Eurycea bislineata and Notophthalmus viridescens). We conducted skin transcriptomics (n = 13), proteomics (n = 91) and AMP database querying to identify candidate AMPs. With candidate AMPs, we identified correlations with the skin microbiome (16S rRNA amplicon) and synthesized 20 peptides to use in challenge assays with pathogens of amphibians (Batrachochytrium dendrobatidis: Bd) and humans (ESKAPEE pathogen panel). Using transcriptomics, candidate AMP genes (30-67 genes) were detected in all individuals with Cathelidicin-like peptides being most common. Using proteomics, detectable AMPs were only found in a subset of salamanders (31/91) - predominately E. bislineata - with Kinin-like peptides being most common. Candidate AMP composition generally predicted skin bacterial composition. Crude and synthesized peptides showed limited activity against Bd. Two synthesized Cathelicidin-like peptides (from P. cinereus) showed moderate to strong killing activity against human pathogens, Acinetobacter baumannii and Escherichia coli. We show that mining under-studied taxa and using multi-omics fuels AMPs discovery, reveals dynamic AMPs-microbial relationships, and informs the therapeutic potential of AMPs usage in conservation and translational applications. [doi:10.25345/C5ZP3WC3C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Salamander ; Antimicrobial peptides ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Carly Muletz-Wolz, Smithsonian Institution, USA
Timothy Cleland, Smithsonian Institution, USA
Submitting User: tpcleland
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