MassIVE MSV000083273

Partial Public PXD012158

proteomics and phosphoprotemics analysis of Ndufs4 KO and wild-type mouse brain upon rapamycin treatment

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Description

+ 2 experiments: - effect of rapamycin in Ndufs4 KO mouse --> 3 groups: WT, KO and KR (KO+RP) - effect of rapamycin in wild-type mouse --> 2 groups: WT and WR (WT+RP) + samples from whole brains homogenized in liquid nitrogen + proteins digested with trypsin. No fractionation + phosphopeptides enriched with IMAC beads + instruments: Orbitrap Q Exactive (for proteome), Orbitrap LTQ Velos (for phosphoproteome) + 90 minutes gradient runs + search and quantification with MaxQuant [doi:10.25345/C57G8J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: brain ; mouse ; mitochondria ; leigh syndrome ; rapamycin ; signalling ; phosphorylation ; respiratory complex I ; ndufs4 ; NADH ubiquinone ; proteome ; phosphoproteome ; PKC ; mTOR ; respiratory chain ; inflammation ; neurodegeneration

Contact

Principal Investigators:
(in alphabetical order) 		Judit Villen, University of Washington, USA
Submitting User: Miguel

Publications

Miguel Martin-Perez, Anthony S. Grillo, Takashi K. Ito, Anthony S. Valente, Jeehae Han, Samuel W. Entwisle, Heather Z. Huang, Dayae Kim, Masanao Yajima, Matt Kaeberlein and Judit Villén.
PKC downregulation upon rapamycin treatment attenuates mitochondrial disease.
Nat Metab 2020 Dec; doi: 10.1038/s42255-020-00319-x (https://www.nature.com/articles/s42255-020-00319-x).

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.