In this study, we have developed a parallel reaction monitoring (PRM)-based assay for quantitative profiling of 83 PTKs. The assay detects 308 proteotypic peptides from 54 receptor tyrosine kinases and 29 nonreceptor tyrosine kinases in a single run. We used this assay to determine PTKs in lung cancer cell lines with either susceptibility (11-18) or acquired resistance (11-18R) to the epidermal growth factor receptor tyrosine kinase inhibitor erlotinib. We also performed label free shotgun proteome analyses of the 11-18 and 11-18R cells and iTRAQ comparisons of phosphotyrosine peptides from 11-18 and 11-18R cells.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: receptor tyrosine kinase
Principal Investigators: (in alphabetical order) |
Daniel Liebler |
Submitting User: | rslebos |
Kim HJ, Lin D, Lee HJ, Li M, Liebler DC.
Quantitative Profiling of Protein Tyrosine Kinases in Human Cancer Cell Lines by Multiplexed Parallel Reaction Monitoring Assays.
Mol. Cell Proteomics. 2016 Feb;15(2):682-91. Epub 2015 Dec 2.
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