Nitrogen starvation results in polyphosphate accumulation in Pseudomonas aeruginosa. Using Percoll gradient centrifugation, we pelleted polyphosphate granules and compared their composition to the unfractionated cell lysate using mass spectrometry. Among the enriched proteins was the alginate regulatory protein AlgP. Its unusual amino-acid composition, overall charge, and physicochemical properties lead us to further investigate its role in granule association and spacing in Pseudomonas aeruginosa.
[doi:10.25345/C5D232]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: starvation ; pseudomonas ; polyphosphate
Principal Investigators: (in alphabetical order) |
James R. Williamson, The Scripps Research Institute, United States Lisa Racki, The Scripps Research Institute, United States |
Submitting User: | patsalo |
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