MassIVE MSV000082505

Complete Public PXD010236

Time-Course Mass Spectrometry Data of Adipose Mesenchymal Stem Cells Acquiring Chondrogenic Phenotype

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Description

Project description: Adipose mesenchymal stem cells (ADSCs) are fast gaining popularity in regenerative medicine for their pluripotency and relative use of collection. Currently, we are addressing the use of ADSCs in otolaryngology, for the treatment of vocal fold (VF) paralysis. VF can result from accidentally sectioning the recurrent laryngeal nerve (RLN) during thyroidectomy. This prevents closure of the upper larynx and promotes food aspiration into the lungs. Volume augmenting materials are sometimes injected in order to bring the paralyzed VF in close proximity to the counter lateral VF. The procedure is called injection medialization laryngoplasty (IML); we have recently developed a rabbit IML model for testing biological reagents in order to improve outcomes. As part of these studies ADSCs were differentiated into chondrocytes and their fate was studied in vitro and in situ. We used mass spectrometry proteomics because microarrays based gene expression platforms do not report actual protein levels. The data show protein levels in controls and chondrogenically differentiating rabbit ADSCs at 0, 14 and 28 days, analyzed using nano-flow liquid chromatography electrospray ionization tandem mass spectrometry (nLC-MS/MS). [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Proteomics ; mass spectrometry ; adipose mesenchymal stem cells ; regenerative medicine ; chondrogenic time-course

Contact

Principal Investigators:
(in alphabetical order) 		Serban San-Marina, Mayo, Rochester, MN, USA
Submitting User: ssanmarina1
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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