MassIVE MSV000090802

Partial Public PXD038455

Identification of proteins binding "ubiquitin like" activity based probes

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Description

HA antibody beads were used to pulldown HA proteins in a cell lysate. The HA protein is an activty based probe (consisting of a small protein and a warhead) and was spiked into the cell lysate. 35, 156, 149 and 61 are different probes. Experiments were performed with and without pretreatment of the cell lysate with IAA an alkylating agent which should reduce the binding between the probe and its targets in cell lysate (cysteine based proteases). [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: activity based probes ; ubiquitin like ; bottom-up proteomics ; affinity enrichment

Contact

Principal Investigators:
(in alphabetical order) 		Petra Janning, MPI of Molecular Physiology, Germany
Submitting User: janning
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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