MassIVE MSV000086254

Partial Public PXD021905

Respiratory vinyl chloride reductive dechlorination to ethene in TceA-expressing Dehalococcoides mccartyi

Description

Organohalide-respiring Dehalococcoidia bacteria are one of the few microorganisms capable of transforming chlorinated solvents to benign ethene in anoxic environments. The tceA gene found in these bacteria, coding the trichloroethene-dechlorinating RDase TceA, is frequently detected in contaminated groundwater but not recognized as a biomarker for vinyl chloride detoxification. Here, we demonstrate that the tceA-carrying Dehalococcoides mccartyi (Dhc) strains FL2 and 195 grow with VC as electron acceptor when sufficient vitamin B12 is provided. Global proteomic profiling confirmed the predominant TceA expression in VC-grown Dhc FL2 cells, providing a line of evidence for the implication of TceA in respiratory VC reductive dechlorination. [doi:10.25345/C5KJ4T] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Dehalococcoides, strain FL2, vinyl chloride, TceA

Contact

Principal Investigators:
(in alphabetical order)
Frank E. Loffler, University of Tennessee-Knoxville, United States of America
Submitting User: mvillal1
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