MassIVE MSV000085289

Partial Public PXD018560

An atlas of O-glycans on peptide hormones reveal wide biological roles

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Description

Peptide hormones and neuropeptides encompass a large class of bioactive peptides that regulate physiological processes like anxiety, blood glucose, appetite, inflammation and blood pressure. Here, we executed a focused discovery strategy to provide an extensive map of O-glycans on peptides hormones. We found that almost one third of the 279 classified peptide hormones carry O-glycans. Many of the identified O-glycosites were conserved and are predicted to serve roles in proprotein processing, receptor interaction, biodistribution and biostability. We demonstrate that O-glycans positioned within the receptor binding motifs of members of the NPY and Glucagon families modulate receptor activation properties and substantially extend peptide half-lives. Our study highlights the importance of O-glycosylation in the biology of peptide hormones, and our map of O-glycosites in this large class of biomolecules serves as a discovery platform for this important class of molecules with potential opportunities for new drug designs. [doi:10.25345/C54D7J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Peptide stability ; Glycosylation ; Metabolism ; GLP-1 ; Secretin ; PYY

Contact

Principal Investigators:
(in alphabetical order) 		Katrine Schjoldager, University of Copenhagen, Institute of Cellular and Molecular Medicine, Copenhagen Center for Glycomics, Denmark
Submitting User: Daugbjerg
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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