In the present study, we have used a quantitative LC-MS/MS approach using total and phosphopeptide-enriched proteins to identify the changes that occur in the proteome and phosphoproteome of MA-10 Leydig cells during both the stimulatory phase (Fsk/cAMP treatment) and inhibitory phase (AICAR-mediated activation of AMPK) of steroidogenesis. The phosphorylation level of several proteins, including some never before described in Leydig cells, was significantly altered during stimulation and inhibition of steroidogenesis. Our data provide new key insights into the finely tuned and dynamic processes that ensure adequate steroid hormone production.
[doi:10.25345/C5C824K2P]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Testis ; Leydig cells ; Steroidogenesis ; Star ; AMPK ; Phosphoproteomics ; Proteomics
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Jacques J. Trembay, CHU de Quebec Universite Laval, Canada |
Submitting User: | JJTremblay01 |
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