HLA peptides from mono-allelic B-cell line using tandem mass-spectrometry
We purchased the monoallelic B-cell line (B721.221), with HLA allele A*2603, directly from the International Histocompatibility Working group (Seattle, Washington). We cultured the cells and isolated HLA peptides from approximately 300 million cells as previously described (Abelin et al. 2017). We identified the isolated HLA peptides using an LC-coupled tandem mass-spectrometer (ThermoFisher, Orbitrap Fusion Lumos) and a reference dataset of a human proteome (Swissprot), applying settings from the literature for analyzing HLA peptides (Bassani-Sternberg et al. 2015) and ProteomeDiscoverer 2.3 software (Thermofisher).
[doi:10.25345/C5XG9FM9X]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: HLA ; Human leukocyte antigen ; single molecule protein sequencing
Principal Investigators: (in alphabetical order) |
Jagannath Swaminathan, Erisyon Inc, United States of America |
Submitting User: | jagannath |
Number of Files: | |
Total Size: | |
Spectra: | |
Subscribers: | |
Owner | Reanalyses | |
---|---|---|
Experimental Design | ||
Conditions:
|
||
Biological Replicates:
|
||
Technical Replicates:
|
||
Identification Results | ||
Proteins (Human, Remapped):
|
||
Proteins (Reported):
|
||
Peptides:
|
||
Variant Peptides:
|
||
PSMs:
|
||
Quantification Results | ||
Differential Proteins:
|
||
Quantified Proteins:
|
||
Browse Dataset Files | Browse Results |
FTP Download Link (click to copy):
|