MassIVE MSV000091811

Partial Public PXD041852

Ablation of ZC3H11A causes early embryonic lethality and dysregulation of metabolic processes

Description

In human somatic cells, ZC3H11A has been recently characterized as an RNA-export protein that functions through its interaction with TREX complex proteins. In order to identify its interacting partners in embryonic cells, and to investigate if ZC3H11A maintains its association with the TREX complex in mouse embryonic stem cells (mESCs), we have performed co-immunoprecipitations (co-IPs) using anti-ZC3H11A, anti-THOC2 and anti-IgG antibodies followed by mass spectrometry (MS) analyses. Statistical analyses of detected MS intensities from the biological replicates (n=4) have revealed a number of proteins with statistically significant interaction with ZC3H11A and THOC2. Results in xlsx format contain the log-fold change in protein intensities in the ZC3H11A co-IP relative to the IgG co-IP and is presented along with the adjusted P-values. [doi:10.25345/C5DJ58S46] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: ZC3H11A, mouse embryonic stem cells, label-free, RNAseq

Contact

Principal Investigators:
(in alphabetical order)
Marten Larsson, Uppsala University, Sweden
Shady Younis, Stanford University, USA
Submitting User: Toto_Marten
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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