Description
In human somatic cells, ZC3H11A has been recently characterized as an RNA-export protein that functions through its interaction with TREX complex proteins. In order to identify its interacting partners in embryonic cells, and to investigate if ZC3H11A maintains its association with the TREX complex in mouse embryonic stem cells (mESCs), we have performed co-immunoprecipitations (co-IPs) using anti-ZC3H11A, anti-THOC2 and anti-IgG antibodies followed by mass spectrometry (MS) analyses. Statistical analyses of detected MS intensities from the biological replicates (n=4) have revealed a number of proteins with statistically significant interaction with ZC3H11A and THOC2. Results in xlsx format contain the log-fold change in protein intensities in the ZC3H11A co-IP relative to the IgG co-IP and is presented along with the adjusted P-values.
[doi:10.25345/C5DJ58S46]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: ZC3H11A, mouse embryonic stem cells, label-free, RNAseq
Contact
Principal Investigators:
(in alphabetical order)
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Marten Larsson, Uppsala University, Sweden
Shady Younis, Stanford University, USA
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Submitting User: |
Toto_Marten
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Identification Results |
Proteins (Human, Remapped):
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Proteins (Reported):
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Peptides:
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PSMs:
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Quantified Proteins:
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Number of distinct conditions across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct condition labels are counted across all files submitted in the "Metadata" category
having a "Condition" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct replicate labels are counted across all files submitted in the "Metadata" category
having a "BioReplicate" or "Replicate" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
associated with this dataset.
The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
considered.
"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.