MassIVE MSV000083581

Complete Public PXD013107

20180725 Buthelezi TripleTOF FFPE of human breast tissue

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Description

Sindisiwe Buthelezi generated these "shotgun" data in July of 2018 on a SCIEX TripleTOF 6600 at the Council for Scientific and Industrial Research in Pretoria, South Africa. The twelve Information-Dependent Acquisition experiments compared one-slide-at-a-time pressure-based paraffin removal (A1-4) with all-at-once paraffin removal (A5-8) with freshly frozen samples (A9-12) using human breast tissue under CSIR Research Ethics Committee reference 213/2017. After conversion to mzML, a total of 474,722 tandem mass spectra were available, of which 39% resulted from doubly-charged precursor ions. Over all twelve experiments, tandem mass spectra were acquired at a rate of 8.40 Hz. MS/MS scans averaged a peak density average of 54 peaks per spectrum. [doi:10.25345/C59323] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: FFPE ; Formalin-fixed paraffin embedded

Contact

Principal Investigators:
(in alphabetical order) 		Stoyan Stoychev, Council for Scientific and Industrial Research, South Africa
Submitting User: dtabb73
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.