In this study, we combined the transmembrane segment-oriented method hpTC with a solid phase enrichment of glycopeptides (SPEG) and standard 'detergent and trypsin' approach into a three-pronged 'Pitchfork' strategy to maximize membrane proteome coverage. The strategy enabled identification of over 1200 IMPs in human lymphoma cells.
[doi:10.25345/C5GS49]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: integral membrane proteins ; cyanogen bromide ; glycopeptide enrichment ; missing proteins ; mantle cell lymphoma
Principal Investigators: (in alphabetical order) |
Ondrej Vit, Charles University, First Faculty of Medicine, Czech Republic |
Submitting User: | hwllffrdd |
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Owner | Reanalyses | |
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