MassIVE MSV000088752

Complete Public PXD031356

The Proteomic Characteristics of Rainbow Trout Blood with Severe, Moderate and Asymptomatic course of Vibrio anguillarum infection

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Description

This data describes the clinical picture of natural Vibrio anguillarum infection in rainbow trout during an outbreak on a fish farm. Molecular mechanisms associated with the host immune response have been investigated using mass spectrometric analysis of trout plasma proteins. Three fish populations were identified among infected trout according to the severity of infection: fish with severe lesions (SL), with moderate infectious process (IP) and asymptomatic fish (AS). As expected, pro-inflammatory interleukins, complement components, acute phase proteins and antimicrobial peptides were implicated in the acute pathogenesis. Systemic coagulopathy was accompanied by increased antithrombotic reactions. Reconstruction of metabolic pathways also revealed a high energy requirement for the immune response in severely affected fish. An unexpected result was a small difference between fish with moderate symptoms and fish with no or minor external signs of pathology, proposed as resistant to infection. Increased production of antiproteases and enhanced blood coagulation cascade were observed in healthier fish, which may underlie the mechanisms of a controlled, non-self-damaging immune response to infection. [doi:10.25345/C5XC6Z] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Rainbow trout ; plasma proteomics ; LFQ ; Vibrio anguillarum ; Shotgun proteomic analysis ; Vibriosis infections

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Principal Investigators:
(in alphabetical order) 		Ekaterina V. Borvinskaya, Institute of Biology of Irkutsk State University, Irkutsk, Russia
Irina V. Sukhovskaya, Institute of Biology of Karelian Research Centre of Russian Academy of Sciences, Petrozavodsk, Russia
Stanislav R. Kurpe, Institute of Protein Research of the Russian Academy of Sciences, Pushchino, Russia
Submitting User: stkurpe
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