MassIVE MSV000094579

Partial Public PXD051612

A proteomic map of the life cycle stages of the spotted lanterfly (Lycorma Delicatula)

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Description

Proteomic samples were harvested from different life cycle stages of the invasive pest Lycorma Delicatula, or the Spotted Lanternfly. Adults were captured in the wild while egg and early instar samples were obtained by hatching insects in captivity. Samples were homogenized by bead disruption after percussive homogenization of samples frozen at -80C. Homogenization in 1x S-trap lysis buffer (Protifi, Long Island, NY) was performed in 30s pulses until homogenization appeared complete by visual inspection. The resulting lysate was reduced in DTT and alkylated with iodoacetamide prior to S-Trap mini digestion following vendor protocols. The resulting peptides were analyzed by diaPASEF based proteomics using the default workflow for "short gradient diaPASEF" in TimsControl 4.0. Raw files were processed with a 6 frame translation of the L. delicatula genome sequence and annotated with EggNog Mapper V2 with peptide match and quantification performed with SpectroNaut 18. Please see the associated metadata excel sheet for sample identities. [doi:10.25345/C5KW57V8M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Spotted lanternfly ; Lycorma delicatula ; Instars ; Invasive lanternfly

Contact

Principal Investigators:
(in alphabetical order) 		Benjamin C Orsburn, Johns Hopkins, USA
Submitting User: ben_orsburn
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.