This dataset consists of 37 raw MS files and associated peak lists and result files, acquired on TripleTOF6600 mass spectrometer operated in Data Dependent Acquisition mode. The files are associated with a manuscript submitted for publication by Segal et al. that determines the protein-protein and proximity interaction landscape of 14-3-3 proteins.
This particular dataset consists in files associated with three baits (14-3-3 interactors) profiled in both a wild type and a 14-3-3 binding site mutant. Each bait was profiled in biological duplicates by BioID (*using the BioID2 enzyme), alongside a total of 25 negative controls (used for analysis with SAINTexpress). See details within the README file.
Samples were generated by Dmitri Segal and affinity purification and mass spectrometry were performed by Zhen-Yuan Lin with oversight from Brett Larsen and Anne-Claude Gingras. Analysis was performed by Anne-Claude Gingras and Mikko Taipale.
Corresponding authors: Mikko Taipale (mikko.taipale@utoronto.ca)/lead author and Anne-Claude Gingras (gingras@lunenfeld.ca). Anne-Claude Gingras should be contacted for questions on this dataset.
[doi:10.25345/C5FJ29H3Q]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: BioID ; Proximity-dependent biotinylation ; 14-3-3 proteins ; P-body ; Stress granules
Principal Investigators: (in alphabetical order) |
Anne-Claude Gingras, LTRI, Canada |
Submitting User: | gingraslab |
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Owner | Reanalyses | |
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Browse Dataset Files | Browse Results |
FTP Download Link (click to copy):
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