MassIVE MSV000087709

Partial Public PXD027019

Vitamin C differentially impacts the serum proteome profile in female and male mice

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Description

Suboptimal blood vitamin C (ascorbate) level increases the risk of several chronic diseases. However, the detection of hypovitaminosis C is not a simple task as ascorbate is unstable in blood samples. In this study, we examined the serum proteome of mice lacking the enzyme gulonolactone oxidase (Gulo) required for the biosynthesis of ascorbate. Gulo-/- mice were supplemented with different concentrations of ascorbate in drinking water and serum was collected to identify proteins correlating with serum ascorbate levels using an LC-MS/MS quantitative proteomic approach. Parallel reaction monitoring was performed to validate the correlations. We uncovered that the serum proteome profiles differ significantly between male and female mice. Also, unlike Gulo-/- males, a four-week treatment of ascorbate did not entirely re-establish the serum proteome profile of ascorbate-deficient Gulo-/- females to the optimal profile exhibited by Gulo-/- females that never experienced an ascorbate deficiency. Finally, serum proteins involved in retinoid metabolism, cholesterol, and lipid transport were similarly affected by ascorbate levels in males and females. In contrast, proteins regulating serum peptidases and protein of the acute phase response were different between males and females. These proteins are potential biomarkers correlating with blood ascorbate levels and require further study in standard clinical settings. [doi:10.25345/C5RR8Z] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: vitamin C, gulonolactone oxidase, mass spectrometry, proteomics, serum, sex related differences, mouse

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Principal Investigators:
(in alphabetical order) 		Michel Lebel, Centre Hospitalier Universite Laval, Canada
Submitting User: MicLeb
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