MassIVE MSV000092331

Partial Public PXD043459

Proteomics Analysis of the UMK2 Mutation in Arabidopsis thaliana seeds

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Description

We compared the proteome of dry as well as germinating seeds of Arabidopsis thaliana wild type Col-0 with the respective proteomes from an umk2 (AT4G25280) frameshift mutant. Germinating seeds were incubated for 48 hours in 1/2-MS medium in Petri dishes at 4 degree Celsius followed by 24 hours of a phytochamber under long-day conditions. Protein extraction, digestion by SP3 workflow, and LC-IMS-MS/MS analysis as described in https://doi.org/10.1038/s41477-022-01308-6. [doi:10.25345/C5697079M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Arabidopsis thaliana ; Seeds ; UMK2 ; timsTOF Pro

Contact

Principal Investigators:
(in alphabetical order) 		Hans-Peter Braun, Plant Genetics, department plant proteomics, Germany
Nils Rugen, Leibniz University Hannover, Germany
Submitting User: Nils_2023

Publications

Rinne J, Niehaus M, Medina-Escobar N, Straube H, Schaarschmidt F, Rugen N, Braun HP, Herde M, Witte CP.
Three Arabidopsis UMP kinases have different roles in pyrimidine nucleotide biosynthesis and (deoxy)CMP salvage.
In: The Plant Cell, 2024;, koae170, https://doi.org/10.1093/plcell/koae170.

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